NLS2091  (novus biologicals)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion

doi: 10.1155/2022/8251503

Figure Lengend Snippet: Effects of STW on the expression levels of GLUT1, MCT4, and GPR81 mRNA in mouse blastocyst tissue: (a) expression levels of GLUT1 mRNA in blastocyst tissues of pregnant mice in each group; (b) expression levels of MCT4 mRNA in blastocyst tissues of pregnant mice in each group; (c) expression levels of GPR81 mRNA in blastocyst tissues of pregnant mice in each group. Compared with the control group, ∗ P < 0.05; compared with the model group, # ∗ P < 0.05; compared with the DQYT group, △ ∗ P < 0.05.

Article Snippet: After incubation with 10% goat serum for 30 min to block nonspecific antigen binding, the tissue sections were incubated with anti-HK2 antibody (Abcam, USA, 1 : 400), anti-PKM2 antibody (Proteintech, China, 1 : 400), anti-LDHA antibody (Abcam, 1 : 200), anti-GLUT1 antibody (Abcam, 1 : 200), anti-MCT4 antibody (Proteintech, 1 : 100), and anti-GPR81 antibody (Novus, USA, 1 : 200) mixed with the TROP2 antibody (bio-techne, USA, 1 : 60) overnight at 4°C, followed by incubation with fluorescently labeled secondary antibodies (Proteintech, 1 : 100) in a humid chamber at 37°C for 1 h in the dark.

Techniques: Expressing